RT info:eu-repo/semantics/article
T1 The Effect of the Environmental Temperature on the Adaptation to Host in the Zoonotic Pathogen Vibrio vulnificus
A1 Sanjuán Caro, Eva
A1 Hernández-Cabanyero, Carla
A1 Fouz, Belén
A1 Pajuelo, David
A1 Vallejos-Vidal, Eva
A1 Reyes-López, Felipe E.
A1 Amaro, Carmen
A2 BioquímicaMicrobiología, Biología Celular y Genética
K1 V. vulnificus
K1 temperature
K1 host adaptation
K1 transcriptome
K1 microarray
AB Vibrio vulnificus is a zoonotic pathogen that lives in temperate, tropical and subtropicalaquatic ecosystems whose geographical distribution is expanding due to globalwarming. The species is genetically variable and only the strains that belong to thezoonotic clonal-complex can cause vibriosis in both humans and fish (being its mainhost the eel). Interestingly, the severity of the vibriosis in the eel and the human dependslargely on the water temperature (highly virulent at 28◦C, avirulent at 20◦C or below)and on the iron content in the blood, respectively. The objective of this work was tounravel the role of temperature in the adaptation to the host through a transcriptomicand phenotypic approach. To this end, we obtained the transcriptome of a zoonoticstrain grown in a minimum medium (CM9) at 20, 25, 28, and 37◦C, and confirmed thetranscriptomic results by RT-qPCR and phenotypic tests. In addition, we comparedthe temperature stimulon with those previously obtained for iron and serum (fromeel and human, respectively). Our results suggest that warm temperatures activateadaptive traits that would prepare the bacteria for host colonization (metabolism, motility,chemotaxis, and the protease activity) and fish septicemia (iron-uptake from transferrinand production of O-antigen of high molecular weight) in a generalized manner, whileenvironmental iron controls the expression of a host-adapted virulent phenotype (toxinsand the production of a protective envelope). Finally, our results confirm that beyond theeffect of temperature on the V. vulnificus distribution in the environment, it also has aneffect on the infectious capability of this pathogen that must be taken into account topredict the real risk of V. vulnificus infection caused by global warming.
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35666
UL http://riull.ull.es/xmlui/handle/915/35666
LA en
NO https://doi.org/10.3389/fmicb.2020.00489
DS Repositorio institucional de la Universidad de La Laguna
RD 29-may-2024