RT info:eu-repo/semantics/article
T1 Alteration of soil rhizobial populations by rabbit latrines could impair symbiotic nitrogen fixation in the insular alpine ecosystem of Teide National Park.
A1 Díaz Peña, Francisco Javier
A1 Pulido Suárez, Laura
A1 Notario del Pino, Jesús
A1 Medina Cabrera, Ayoze
A1 León Barrios, Milagros
K1 Legume
K1 Rhizobia
K1 Nitrogen-fixation
K1 Rabbits
K1 Alpine ecosystem
K1 Oceanic islands
AB Spartocytisus supranubius (Teide broom) is the dominant legume of the high-mountain ecosystem in Teide National Park (Tenerife, Canary Islands). Herbivory by rabbits is one important cause of its declining populations.Biological nitrogen-fixing of rhizobia in symbiosis with S. supranubius is hypothesized to be the main nitrogen (N) input in this alpine ecosystem. Therefore, our main objective was to investigate whether large rabbit droppingaggregations (latrines) affect the S. supranubius-rhizobia symbiosis by disturbing the rhizobia populations in the soils. Soils were sampled in the rhizosphere of broom plants without (RS) and with latrines (RLS) as well as in non-rhizospheric bare areas without (BS) and with latrines (BLS), and assessed for N content and number of rhizobia. Rhizobial diversity was evaluated by sequencing three housekeeping genes in root-nodule bacteria isolated from broom seedlings grown in these soils. Results showed that BS had the lowest rhizobia counts and N content, whereas RS had on average the highest, which were not exceeded in soils with N-supply from latrines (BLS and RLS). Phylogenetic analysis revealed that strains from the Bradyrhizobium canariense/Bradyrhizobiumlupini lineage are the main microsymbionts of S. supranubius. Bradyrhizobium rifense/Bradyrhizobium cytisi strains were scarce and only detected in latrine influenced soils, where endophytic Bosea strains seem a common cohabitant in nodules. It can be concluded that S. supranubius-Bradyrhizobia symbiosis is a key N input in Teide soils and that rabbit latrines may affect the N-fixing symbiosis by altering abundance and diversity of the soilrhizobial populations. Moreover, latrines could also interfere in symbiosis by favouring co-entrance of nonnodulating bacteria inside the root nodules.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35963
UL http://riull.ull.es/xmlui/handle/915/35963
LA en
NO https://doi.org/10.1016/j.apsoil.2020.103850
DS Repositorio institucional de la Universidad de La Laguna
RD 15-may-2024