RT info:eu-repo/semantics/article
T1 Correlation between EGFR amplification and the  expression of microRNA-200c in primary Glioblastoma multiforme
A1 Serna, Eva
A1 López Ginés, Concha
A1 Monleón, Daniel
A1 Muñoz-Hidalgo, Lisandra
A1 Callaghan, Robert C.
A1 Gil-Benso, Rosario
A1 Martinetto, Horacio
A1 Gregori-Romero, María Aurelia
A1 González-Darder, José Manuel
A1 Cerdá-Nicolás, Miguel J.
K1 Primary glioblastoma multiforme
K1 EGFR
K1 EGFR amplification
K1 The expression of microRNA-200c
K1 MicroRNA-200c
AB Extensive infiltration of the surrounding healthy brain tissue is a critical feature in glioblastoma. Several miRNAs have been related to gliomagenesis, some of them related with the EGFR pathway. We have evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns, studied by fluorescence in situ hybridization in tissue microarrays, of 30 cases of primary glioblastoma multiforme, whose clinicopathological and immunohistochemical features have also been analyzed. MicroRNA-200c showed a very significant difference between tumors having or not EGFR amplification. This microRNA plays an important role in epithelial-mesenchymal transition, but its implication in the behavior of glioblastoma is largely unknown. With respect to EGFR status our cases were categorized into three groups: high level EGFR amplification, low level EGFR amplification, and no EGFR amplification. Our results showed that microRNA-200c and E-cadherin expression are down-regulated, while ZEB1 is up-regulated, when tumors showed a high level of EGFR amplification. Conversely, ZEB1 mRNA expression levels were significantly lower in the group of tumors without EGFR amplification. Tumors with a low level of EGFR amplification showed ZEB1 expression levels comparable to those detected in the group with a high level of amplification. In this study we provide what is to our knowledge the first report of association between microRNA-200c and EGFR amplification in glioblastomas.
SN 1932-6203
YR 2014
FD 2014
LK http://riull.ull.es/xmlui/handle/915/36190
UL http://riull.ull.es/xmlui/handle/915/36190
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 15-may-2024