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dc.contributor.authorValenzuela Fernández, Agustín 
dc.contributor.authorMuñoz Barrera, Adrián
dc.contributor.authorCiuffreda, Laura
dc.contributor.authorAlcoba Florez, Julia
dc.contributor.authorRubio Rodríguez, Luis A.
dc.contributor.authorRodríguez Pérez, Héctor
dc.contributor.authorGil Campesino, Helena
dc.contributor.authorGarcía Martínez de Artola, Diego
dc.contributor.authorSalas Hernández, Josmar 
dc.contributor.authorRodríguez Núñez, Julia 
dc.contributor.authorÍñigo Campos , Antonio
dc.contributor.authorGarcía Olivares, Víctor
dc.contributor.authorDíez Gil, Oscar
dc.contributor.authorGonzález Montelongo, Rafaela
dc.contributor.authorLorenzo Salazar, José M.
dc.contributor.authorFlores, Carlos
dc.date.accessioned2024-01-15T21:05:41Z
dc.date.available2024-01-15T21:05:41Z
dc.date.issued2023
dc.identifier.issn2001-0370
dc.identifier.urihttp://riull.ull.es/xmlui/handle/915/35320
dc.description.abstractOn July 23, 2022, monkeypox disease (mpox) was declared a Public Emergency of International Concern (PHEIC) by the World Health Organization (WHO) due to a multicountry outbreak. In Europe, several cases of mpox virus (MPXV) infection related to this outbreak were detected in the Canary Islands (Spain). Here we describe the combination of viral DNA sequencing and bioinformatic approaches, including methods for de novo genome assembly and short- and long-read technologies, used to reconstruct the first MPXV genome isolated in the Canary Islands on the 31st of May 2022 from a male adult patient with mild symptoms. The same sequencing and bioinformatic approaches were then validated with three other positive cases of MPXV infection from the same mpox outbreak. We obtained the best results using a reference-based approach with short reads, evidencing 46–79 nucleotide variants against viral sequences from the 2018–2019 mpox outbreak and placing the viral sequences in the new B.1 sublineage of clade IIb of the MPXV classification. This study of MPXV demonstrates the potential of metagenomics sequencing for rapid and precise pathogen identification.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.relation.ispartofseriesComputational and Structural Biotechnology Journal, V. 21, pp. 2197-2203, 2023
dc.rightsLicencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
dc.titleBioinformatic approaches to draft the viral genome sequence of Canary Islands cases related to the multicountry mpox virus 2022-outbreak.
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1016/j.csbj.2023.03.020
dc.subject.keywordBioinformatics
dc.subject.keywordViral surveillance
dc.subject.keywordGenome assembly


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Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
Except where otherwise noted, this item's license is described as Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)